Serine/Threonine Protein Phosphatase Assays
نویسندگان
چکیده
منابع مشابه
Protein phosphatase PP4
in Greek mythology, one of Hercules’ labors was to slay the Hydra. The task seems impossible, as the creature was a multi-headed monster with the ability to grow two new heads when one was cleaved off. Oncologists face a similar daunting task in eliminating malignant cells, as each cancer appears to have its own Hydra protecting it. The suppression of the Forkhead box class O (FOXO) family of t...
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Epidermal growth factor stimulates a cyclic AMPindependent protein kinase associated with membrane vesicles derived from human epidermoid carcinoma cells (Carpenter, G., King, L., Jr., and Cohen, S. (1979) J. Biol. C h m 254,4884-4891). The kinase specifically phosphorylates tyrosyl residues in aM, = 150,000 membrane protein (Ushiro, H., and Cohen, S. (1980) J. Biol. Chem. 255,8363-8365). We sh...
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The major secreted isoenzyme of human prostatic acid phosphatase (PAcP) (EC 3.1.3.2), which catalyses p-nitrophenyl phosphate (PNPP) hydrolysis at acid pH values, was found to have phosphotyrosyl protein phosphatase activity since it dephosphorylated three different phosphotyrosine-containing protein substrates. Several lines of evidence are presented to show that the phosphotyrosyl phosphatase...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2010
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb1818s92